Identification of Novel Two-partner Secretion Family in Burkholderia pseudomallei
Annapoorna Nimaggadda1, Sheila Nathan2, Rahmah Mohamed
1anulins@yahoo.com, Universiti Kebangsaan Malaysia; 2sheila@pkrisc.cc.ukm.my, Universiti Kebangsaan Malaysia
Identification of Novel Two-partner Secretion Family in Burkholderia pseudomallei
Annapoorna Nimaggadda1, Sheila Nathan2, Rahmah Mohamed3
1anulins@yahoo.com, Interim Laboratory, National Institute for Genomics and Molecular Biology, Bangi, Malaysia; 2sheila@pkrisc.cc.ukm.my, Interim Laboratory, National Institute for Genomics and Molecular Biology, Bangi, Malaysia; 3 ram@cgat.ukm.my, Interim Laboratory, National Institute for Genomics and Molecular Biology, Bangi, Malaysia
Probable filamentous hemagglutinin (FhaB) and its transporter (FhaC) of Burkholderia pseuodomallei are believed to have a role in respiratory manifestations of melioidosis. Sequence analysis of the genome of Burkholderia pseuodomallei, K96243, a clinical isolate (sequenced by the Sanger Centre, U. Kingdom) by ARTEMIS identified FhaB and FhaC within an operon and they belong to the Two Partner Secretion (TPS) family. Analysis of FhaB N-terminus homologue revealed an NPNL motif recognised by FhaC to transport it into the external milieu. Phylogenetic analysis using the N-J method clusters the FhaC with the FhaC of Bordetella perstussis confirming its identity functionally. Cloning the FhaB and FhaC homologues would give a basis for the molecular characterization of the TPS family and an insight into its adhesion properties in Burkholderia pseudomallei.